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bovine non o157 h7 e coli strains  (ATCC)


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    ATCC bovine non o157 h7 e coli strains
    Virulence factors identified in twenty-one genetically distinct bovine non-O157:H7 <t>Escherichia</t> <t>coli</t> strains. Virulence factors were identified using Abricate (v1.0.1) to query the virulence factor database (VFDB) and generate virulence profiles for each isolate. Blue cells denote a virulence factor presence while gray cells denote a virulence factor absence. Red cells denote the presence of stx2A and stx2B , genes required for production of Shiga toxin.
    Bovine Non O157 H7 E Coli Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 834 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+o157+e+coli/Escherichia+coli+(Migula)+Castellani+and+Chalmers/pmc12735539-295-7-15
    Average 96 stars, based on 834 article reviews
    bovine non o157 h7 e coli strains - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle"

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    Journal: Microorganisms

    doi: 10.3390/microorganisms13122811

    Virulence factors identified in twenty-one genetically distinct bovine non-O157:H7 Escherichia coli strains. Virulence factors were identified using Abricate (v1.0.1) to query the virulence factor database (VFDB) and generate virulence profiles for each isolate. Blue cells denote a virulence factor presence while gray cells denote a virulence factor absence. Red cells denote the presence of stx2A and stx2B , genes required for production of Shiga toxin.
    Figure Legend Snippet: Virulence factors identified in twenty-one genetically distinct bovine non-O157:H7 Escherichia coli strains. Virulence factors were identified using Abricate (v1.0.1) to query the virulence factor database (VFDB) and generate virulence profiles for each isolate. Blue cells denote a virulence factor presence while gray cells denote a virulence factor absence. Red cells denote the presence of stx2A and stx2B , genes required for production of Shiga toxin.

    Techniques Used:

    Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.
    Figure Legend Snippet: Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

    Techniques Used: Activity Assay, In Vitro, Inhibition

    Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).
    Figure Legend Snippet: Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

    Techniques Used:

    Bar chart of E. coli O157:H7 ATCC 700728 counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of non-O157:H7 E. coli strains. E. coli O157:H7 ATCC 700728 counts were assessed after 24 h of co-incubation with HC or LC consortia under aerobic and anaerobic conditions. ATCC 700728 counts were determined on HardyCHROM O157 chromogenic agar plates. Three biological replicates were conducted for each competition assay under each set of conditions. Colony-forming units (CFU)/mL values were calculated, log 10 -transformed, and statistically compared (t.test) in R using the dplyr (v1.1.4) and ggpubr (v0.6.0) packages. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; “ns” indicates p > 0.05.
    Figure Legend Snippet: Bar chart of E. coli O157:H7 ATCC 700728 counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of non-O157:H7 E. coli strains. E. coli O157:H7 ATCC 700728 counts were assessed after 24 h of co-incubation with HC or LC consortia under aerobic and anaerobic conditions. ATCC 700728 counts were determined on HardyCHROM O157 chromogenic agar plates. Three biological replicates were conducted for each competition assay under each set of conditions. Colony-forming units (CFU)/mL values were calculated, log 10 -transformed, and statistically compared (t.test) in R using the dplyr (v1.1.4) and ggpubr (v0.6.0) packages. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; “ns” indicates p > 0.05.

    Techniques Used: Incubation, Competitive Binding Assay, Transformation Assay

    Related Articles

    Plasmid Preparation:

    Article Title: Supplement A, July 2016
    Article Snippet: Special Symposium ................................................................................................................................................................ 5 Symposium ........................................................................................................................................................................... 7 Roundtable ............................................................................................................................................................................ 27 ILSI Symposium Series on Food Microbiology .................................................................................................................... 31 Technical ................................................................................................................................................................................ 37 Poster ..................................................................................................................................................................................... 83 Author and Presenter Index ............................................................................................................................................. 263 Developing Scientist Competitors .................................................................................................................................... 289 Undergraduate Student Competitors ............................................................................................................................. 291 Journal of Food Protection® ISSN 0362-028X Official Publication

    Transformation Assay:

    Article Title: Supplement A, July 2016
    Article Snippet: Special Symposium ................................................................................................................................................................ 5 Symposium ........................................................................................................................................................................... 7 Roundtable ............................................................................................................................................................................ 27 ILSI Symposium Series on Food Microbiology .................................................................................................................... 31 Technical ................................................................................................................................................................................ 37 Poster ..................................................................................................................................................................................... 83 Author and Presenter Index ............................................................................................................................................. 263 Developing Scientist Competitors .................................................................................................................................... 289 Undergraduate Student Competitors ............................................................................................................................. 291 Journal of Food Protection® ISSN 0362-028X Official Publication

    Bacteria:

    Article Title: Multi-mode separation for target detection and cell growth monitoring
    Article Snippet: .. An example procedure of what is depicted in FIG. 5 is as follows: First, 1.0 mL of appropriately diluted bacteria (E. coli O157:H7 (ATCC 35150), non-O157 E. coli (ATCC 25922), or S. aureus (ATCC 29213)) is mixed with 20 μL of anti-E. coli O157 antibody-coated magnetic beads (Invitrogen #710-03), followed by 100 μL of 10 μm, anti-E. coli O157 coated buoyant silica beads. ..

    Article Title: Multi-mode separation for target detection and cell growth monitoring
    Article Snippet: .. An example procedure of what is depicted in FIG. 5 is as follows: First, 1.0 mL of appropriately diluted bacteria (E. coli O157:H7 (ATCC 35150), non-O157 E. coli (ATCC 25922), or S. aureus (ATCC 29213)) is mixed with 20 μL of anti-E. coli O157 antibody-coated magnetic beads (Invitrogen #710-03), followed by 100 μL of 10 μm, anti-E. coli O157 coated buoyant silica beads. ..

    Article Title: Multi-mode separation for target detection
    Article Snippet: .. An example procedure of what is depicted in FIG. 5 is as follows: First, 1.0 mL of appropriately diluted bacteria (E. coli O157:H7 (ATCC 35150), non-O157 E. coli (ATCC 25922), or S. aureus (ATCC 29213)) is mixed with 20 μL of anti-E. coli O157 antibody-coated magnetic beads (Invitrogen #710-03), followed by 100 μL of 10 μm, anti-E. coli O157 coated buoyant silica beads. ..

    Article Title: Multi-mode separation for target detection
    Article Snippet: .. An example procedure of what is depicted in FIG. 4 is as follows: First, 1 mL of appropriately diluted bacteria (E. coli O157:H7 (ATCC 35150), non-O157 E. coli (ATCC 25922), or S. aureus (ATCC 29213)) is mixed with 20 μL of anti-E. coli O157 antibody-coated magnetic beads (Invitrogen #710-03). ..

    Article Title: Multi-mode separation for target detection and cell growth monitoring
    Article Snippet: .. An example procedure of what is depicted in FIG. 4 is as follows: First, 1 mL of appropriately diluted bacteria (E. coli O157:H7 (ATCC 35150), non-O157 E. coli (ATCC 25922), or S. aureus (ATCC 29213)) is mixed with 20 μL of anti-E. coli O157 antibody-coated magnetic beads (Invitrogen #710-03). ..

    Article Title: Multi-mode separation for target detection and cell growth monitoring
    Article Snippet: .. An example procedure of what is depicted in FIG. 4 is as follows: First, 1 mL of appropriately diluted bacteria (E. coli O157:H7 (ATCC 35150), non-O157 E. coli (ATCC 25922), or S. aureus (ATCC 29213)) is mixed with 20 μL of anti-E. coli O157 antibody-coated magnetic beads (Invitrogen #710-03). ..

    Magnetic Beads:

    Article Title: Multi-mode separation for target detection and cell growth monitoring
    Article Snippet: .. An example procedure of what is depicted in FIG. 5 is as follows: First, 1.0 mL of appropriately diluted bacteria (E. coli O157:H7 (ATCC 35150), non-O157 E. coli (ATCC 25922), or S. aureus (ATCC 29213)) is mixed with 20 μL of anti-E. coli O157 antibody-coated magnetic beads (Invitrogen #710-03), followed by 100 μL of 10 μm, anti-E. coli O157 coated buoyant silica beads. ..

    Article Title: Multi-mode separation for target detection and cell growth monitoring
    Article Snippet: .. An example procedure of what is depicted in FIG. 5 is as follows: First, 1.0 mL of appropriately diluted bacteria (E. coli O157:H7 (ATCC 35150), non-O157 E. coli (ATCC 25922), or S. aureus (ATCC 29213)) is mixed with 20 μL of anti-E. coli O157 antibody-coated magnetic beads (Invitrogen #710-03), followed by 100 μL of 10 μm, anti-E. coli O157 coated buoyant silica beads. ..

    Article Title: Multi-mode separation for target detection
    Article Snippet: .. An example procedure of what is depicted in FIG. 5 is as follows: First, 1.0 mL of appropriately diluted bacteria (E. coli O157:H7 (ATCC 35150), non-O157 E. coli (ATCC 25922), or S. aureus (ATCC 29213)) is mixed with 20 μL of anti-E. coli O157 antibody-coated magnetic beads (Invitrogen #710-03), followed by 100 μL of 10 μm, anti-E. coli O157 coated buoyant silica beads. ..

    Article Title: Multi-mode separation for target detection
    Article Snippet: .. An example procedure of what is depicted in FIG. 4 is as follows: First, 1 mL of appropriately diluted bacteria (E. coli O157:H7 (ATCC 35150), non-O157 E. coli (ATCC 25922), or S. aureus (ATCC 29213)) is mixed with 20 μL of anti-E. coli O157 antibody-coated magnetic beads (Invitrogen #710-03). ..

    Article Title: Multi-mode separation for target detection and cell growth monitoring
    Article Snippet: .. An example procedure of what is depicted in FIG. 4 is as follows: First, 1 mL of appropriately diluted bacteria (E. coli O157:H7 (ATCC 35150), non-O157 E. coli (ATCC 25922), or S. aureus (ATCC 29213)) is mixed with 20 μL of anti-E. coli O157 antibody-coated magnetic beads (Invitrogen #710-03). ..

    Article Title: Multi-mode separation for target detection and cell growth monitoring
    Article Snippet: .. An example procedure of what is depicted in FIG. 4 is as follows: First, 1 mL of appropriately diluted bacteria (E. coli O157:H7 (ATCC 35150), non-O157 E. coli (ATCC 25922), or S. aureus (ATCC 29213)) is mixed with 20 μL of anti-E. coli O157 antibody-coated magnetic beads (Invitrogen #710-03). ..



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    Image Search Results


    Virulence factors identified in twenty-one genetically distinct bovine non-O157:H7 Escherichia coli strains. Virulence factors were identified using Abricate (v1.0.1) to query the virulence factor database (VFDB) and generate virulence profiles for each isolate. Blue cells denote a virulence factor presence while gray cells denote a virulence factor absence. Red cells denote the presence of stx2A and stx2B , genes required for production of Shiga toxin.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Virulence factors identified in twenty-one genetically distinct bovine non-O157:H7 Escherichia coli strains. Virulence factors were identified using Abricate (v1.0.1) to query the virulence factor database (VFDB) and generate virulence profiles for each isolate. Blue cells denote a virulence factor presence while gray cells denote a virulence factor absence. Red cells denote the presence of stx2A and stx2B , genes required for production of Shiga toxin.

    Article Snippet: Heatmap of DRAM metabolic annotations of unique bovine non-O157:H7 E. coli strains and O157:H7 strains (ATCC 43888 and ATCC 700728); Figure S6.

    Techniques:

    Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

    Article Snippet: Heatmap of DRAM metabolic annotations of unique bovine non-O157:H7 E. coli strains and O157:H7 strains (ATCC 43888 and ATCC 700728); Figure S6.

    Techniques: Activity Assay, In Vitro, Inhibition

    Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

    Article Snippet: Heatmap of DRAM metabolic annotations of unique bovine non-O157:H7 E. coli strains and O157:H7 strains (ATCC 43888 and ATCC 700728); Figure S6.

    Techniques:

    Bar chart of E. coli O157:H7 ATCC 700728 counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of non-O157:H7 E. coli strains. E. coli O157:H7 ATCC 700728 counts were assessed after 24 h of co-incubation with HC or LC consortia under aerobic and anaerobic conditions. ATCC 700728 counts were determined on HardyCHROM O157 chromogenic agar plates. Three biological replicates were conducted for each competition assay under each set of conditions. Colony-forming units (CFU)/mL values were calculated, log 10 -transformed, and statistically compared (t.test) in R using the dplyr (v1.1.4) and ggpubr (v0.6.0) packages. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; “ns” indicates p > 0.05.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Bar chart of E. coli O157:H7 ATCC 700728 counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of non-O157:H7 E. coli strains. E. coli O157:H7 ATCC 700728 counts were assessed after 24 h of co-incubation with HC or LC consortia under aerobic and anaerobic conditions. ATCC 700728 counts were determined on HardyCHROM O157 chromogenic agar plates. Three biological replicates were conducted for each competition assay under each set of conditions. Colony-forming units (CFU)/mL values were calculated, log 10 -transformed, and statistically compared (t.test) in R using the dplyr (v1.1.4) and ggpubr (v0.6.0) packages. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; “ns” indicates p > 0.05.

    Article Snippet: Heatmap of DRAM metabolic annotations of unique bovine non-O157:H7 E. coli strains and O157:H7 strains (ATCC 43888 and ATCC 700728); Figure S6.

    Techniques: Incubation, Competitive Binding Assay, Transformation Assay

    Virulence factors identified in twenty-one genetically distinct bovine non-O157:H7 Escherichia coli strains. Virulence factors were identified using Abricate (v1.0.1) to query the virulence factor database (VFDB) and generate virulence profiles for each isolate. Blue cells denote a virulence factor presence while gray cells denote a virulence factor absence. Red cells denote the presence of stx2A and stx2B , genes required for production of Shiga toxin.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Virulence factors identified in twenty-one genetically distinct bovine non-O157:H7 Escherichia coli strains. Virulence factors were identified using Abricate (v1.0.1) to query the virulence factor database (VFDB) and generate virulence profiles for each isolate. Blue cells denote a virulence factor presence while gray cells denote a virulence factor absence. Red cells denote the presence of stx2A and stx2B , genes required for production of Shiga toxin.

    Article Snippet: Bovine non-STEC isolate counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of unique bovine non-O157:H7 E. coli strains against E. coli O157:H7 ATCC 700728.

    Techniques:

    Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

    Article Snippet: Bovine non-STEC isolate counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of unique bovine non-O157:H7 E. coli strains against E. coli O157:H7 ATCC 700728.

    Techniques: Activity Assay, In Vitro, Inhibition

    Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

    Article Snippet: Bovine non-STEC isolate counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of unique bovine non-O157:H7 E. coli strains against E. coli O157:H7 ATCC 700728.

    Techniques:

    Bar chart of E. coli O157:H7 ATCC 700728 counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of non-O157:H7 E. coli strains. E. coli O157:H7 ATCC 700728 counts were assessed after 24 h of co-incubation with HC or LC consortia under aerobic and anaerobic conditions. ATCC 700728 counts were determined on HardyCHROM O157 chromogenic agar plates. Three biological replicates were conducted for each competition assay under each set of conditions. Colony-forming units (CFU)/mL values were calculated, log 10 -transformed, and statistically compared (t.test) in R using the dplyr (v1.1.4) and ggpubr (v0.6.0) packages. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; “ns” indicates p > 0.05.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Bar chart of E. coli O157:H7 ATCC 700728 counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of non-O157:H7 E. coli strains. E. coli O157:H7 ATCC 700728 counts were assessed after 24 h of co-incubation with HC or LC consortia under aerobic and anaerobic conditions. ATCC 700728 counts were determined on HardyCHROM O157 chromogenic agar plates. Three biological replicates were conducted for each competition assay under each set of conditions. Colony-forming units (CFU)/mL values were calculated, log 10 -transformed, and statistically compared (t.test) in R using the dplyr (v1.1.4) and ggpubr (v0.6.0) packages. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; “ns” indicates p > 0.05.

    Article Snippet: Bovine non-STEC isolate counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of unique bovine non-O157:H7 E. coli strains against E. coli O157:H7 ATCC 700728.

    Techniques: Incubation, Competitive Binding Assay, Transformation Assay

    Virulence factors identified in twenty-one genetically distinct bovine non-O157:H7 Escherichia coli strains. Virulence factors were identified using Abricate (v1.0.1) to query the virulence factor database (VFDB) and generate virulence profiles for each isolate. Blue cells denote a virulence factor presence while gray cells denote a virulence factor absence. Red cells denote the presence of stx2A and stx2B , genes required for production of Shiga toxin.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Virulence factors identified in twenty-one genetically distinct bovine non-O157:H7 Escherichia coli strains. Virulence factors were identified using Abricate (v1.0.1) to query the virulence factor database (VFDB) and generate virulence profiles for each isolate. Blue cells denote a virulence factor presence while gray cells denote a virulence factor absence. Red cells denote the presence of stx2A and stx2B , genes required for production of Shiga toxin.

    Article Snippet: ATCC 700728 and ATCC 43888, two E. coli O157:H7 non-Shiga toxin-encoding reference strains, were obtained from the American Type Culture Collection (ATCC; Gaithersburg, MD, USA) and used as O157:H7 indicator strains.

    Techniques:

    Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

    Article Snippet: ATCC 700728 and ATCC 43888, two E. coli O157:H7 non-Shiga toxin-encoding reference strains, were obtained from the American Type Culture Collection (ATCC; Gaithersburg, MD, USA) and used as O157:H7 indicator strains.

    Techniques: Activity Assay, In Vitro, Inhibition

    Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

    Article Snippet: ATCC 700728 and ATCC 43888, two E. coli O157:H7 non-Shiga toxin-encoding reference strains, were obtained from the American Type Culture Collection (ATCC; Gaithersburg, MD, USA) and used as O157:H7 indicator strains.

    Techniques:

    Bar chart of E. coli O157:H7 ATCC 700728 counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of non-O157:H7 E. coli strains. E. coli O157:H7 ATCC 700728 counts were assessed after 24 h of co-incubation with HC or LC consortia under aerobic and anaerobic conditions. ATCC 700728 counts were determined on HardyCHROM O157 chromogenic agar plates. Three biological replicates were conducted for each competition assay under each set of conditions. Colony-forming units (CFU)/mL values were calculated, log 10 -transformed, and statistically compared (t.test) in R using the dplyr (v1.1.4) and ggpubr (v0.6.0) packages. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; “ns” indicates p > 0.05.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Bar chart of E. coli O157:H7 ATCC 700728 counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of non-O157:H7 E. coli strains. E. coli O157:H7 ATCC 700728 counts were assessed after 24 h of co-incubation with HC or LC consortia under aerobic and anaerobic conditions. ATCC 700728 counts were determined on HardyCHROM O157 chromogenic agar plates. Three biological replicates were conducted for each competition assay under each set of conditions. Colony-forming units (CFU)/mL values were calculated, log 10 -transformed, and statistically compared (t.test) in R using the dplyr (v1.1.4) and ggpubr (v0.6.0) packages. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; “ns” indicates p > 0.05.

    Article Snippet: ATCC 700728 and ATCC 43888, two E. coli O157:H7 non-Shiga toxin-encoding reference strains, were obtained from the American Type Culture Collection (ATCC; Gaithersburg, MD, USA) and used as O157:H7 indicator strains.

    Techniques: Incubation, Competitive Binding Assay, Transformation Assay

    Virulence factors identified in twenty-one genetically distinct bovine non-O157:H7 Escherichia coli strains. Virulence factors were identified using Abricate (v1.0.1) to query the virulence factor database (VFDB) and generate virulence profiles for each isolate. Blue cells denote a virulence factor presence while gray cells denote a virulence factor absence. Red cells denote the presence of stx2A and stx2B , genes required for production of Shiga toxin.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Virulence factors identified in twenty-one genetically distinct bovine non-O157:H7 Escherichia coli strains. Virulence factors were identified using Abricate (v1.0.1) to query the virulence factor database (VFDB) and generate virulence profiles for each isolate. Blue cells denote a virulence factor presence while gray cells denote a virulence factor absence. Red cells denote the presence of stx2A and stx2B , genes required for production of Shiga toxin.

    Article Snippet: Cell-free supernatants for all non-O157:H7 E. coli strains were tested in quadruplicate using ATCC 700728 and ATCC 43888 as indicator strains in separate experiments.

    Techniques:

    Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

    Article Snippet: Cell-free supernatants for all non-O157:H7 E. coli strains were tested in quadruplicate using ATCC 700728 and ATCC 43888 as indicator strains in separate experiments.

    Techniques: Activity Assay, In Vitro, Inhibition

    Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

    Article Snippet: Cell-free supernatants for all non-O157:H7 E. coli strains were tested in quadruplicate using ATCC 700728 and ATCC 43888 as indicator strains in separate experiments.

    Techniques:

    Bar chart of E. coli O157:H7 ATCC 700728 counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of non-O157:H7 E. coli strains. E. coli O157:H7 ATCC 700728 counts were assessed after 24 h of co-incubation with HC or LC consortia under aerobic and anaerobic conditions. ATCC 700728 counts were determined on HardyCHROM O157 chromogenic agar plates. Three biological replicates were conducted for each competition assay under each set of conditions. Colony-forming units (CFU)/mL values were calculated, log 10 -transformed, and statistically compared (t.test) in R using the dplyr (v1.1.4) and ggpubr (v0.6.0) packages. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; “ns” indicates p > 0.05.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Bar chart of E. coli O157:H7 ATCC 700728 counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of non-O157:H7 E. coli strains. E. coli O157:H7 ATCC 700728 counts were assessed after 24 h of co-incubation with HC or LC consortia under aerobic and anaerobic conditions. ATCC 700728 counts were determined on HardyCHROM O157 chromogenic agar plates. Three biological replicates were conducted for each competition assay under each set of conditions. Colony-forming units (CFU)/mL values were calculated, log 10 -transformed, and statistically compared (t.test) in R using the dplyr (v1.1.4) and ggpubr (v0.6.0) packages. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; “ns” indicates p > 0.05.

    Article Snippet: Cell-free supernatants for all non-O157:H7 E. coli strains were tested in quadruplicate using ATCC 700728 and ATCC 43888 as indicator strains in separate experiments.

    Techniques: Incubation, Competitive Binding Assay, Transformation Assay

    Virulence factors identified in twenty-one genetically distinct bovine non-O157:H7 Escherichia coli strains. Virulence factors were identified using Abricate (v1.0.1) to query the virulence factor database (VFDB) and generate virulence profiles for each isolate. Blue cells denote a virulence factor presence while gray cells denote a virulence factor absence. Red cells denote the presence of stx2A and stx2B , genes required for production of Shiga toxin.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Virulence factors identified in twenty-one genetically distinct bovine non-O157:H7 Escherichia coli strains. Virulence factors were identified using Abricate (v1.0.1) to query the virulence factor database (VFDB) and generate virulence profiles for each isolate. Blue cells denote a virulence factor presence while gray cells denote a virulence factor absence. Red cells denote the presence of stx2A and stx2B , genes required for production of Shiga toxin.

    Article Snippet: Barcharts of bovine non-O157:H7 E. coli strain counts (log 10 CFU/mL) in competition assays of high-competitive (HC) and low-competitive (LC) consortia of unique bovine non-O157:H7 strains against E. coli O157:H7 ATCC 700728; Table S1.

    Techniques:

    Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.

    Article Snippet: Barcharts of bovine non-O157:H7 E. coli strain counts (log 10 CFU/mL) in competition assays of high-competitive (HC) and low-competitive (LC) consortia of unique bovine non-O157:H7 strains against E. coli O157:H7 ATCC 700728; Table S1.

    Techniques: Activity Assay, In Vitro, Inhibition

    Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).

    Article Snippet: Barcharts of bovine non-O157:H7 E. coli strain counts (log 10 CFU/mL) in competition assays of high-competitive (HC) and low-competitive (LC) consortia of unique bovine non-O157:H7 strains against E. coli O157:H7 ATCC 700728; Table S1.

    Techniques:

    Bar chart of E. coli O157:H7 ATCC 700728 counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of non-O157:H7 E. coli strains. E. coli O157:H7 ATCC 700728 counts were assessed after 24 h of co-incubation with HC or LC consortia under aerobic and anaerobic conditions. ATCC 700728 counts were determined on HardyCHROM O157 chromogenic agar plates. Three biological replicates were conducted for each competition assay under each set of conditions. Colony-forming units (CFU)/mL values were calculated, log 10 -transformed, and statistically compared (t.test) in R using the dplyr (v1.1.4) and ggpubr (v0.6.0) packages. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; “ns” indicates p > 0.05.

    Journal: Microorganisms

    Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle

    doi: 10.3390/microorganisms13122811

    Figure Lengend Snippet: Bar chart of E. coli O157:H7 ATCC 700728 counts (log 10 CFU/mL) in competition assays with high-competitive (HC) and low-competitive (LC) consortia of non-O157:H7 E. coli strains. E. coli O157:H7 ATCC 700728 counts were assessed after 24 h of co-incubation with HC or LC consortia under aerobic and anaerobic conditions. ATCC 700728 counts were determined on HardyCHROM O157 chromogenic agar plates. Three biological replicates were conducted for each competition assay under each set of conditions. Colony-forming units (CFU)/mL values were calculated, log 10 -transformed, and statistically compared (t.test) in R using the dplyr (v1.1.4) and ggpubr (v0.6.0) packages. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001; “ns” indicates p > 0.05.

    Article Snippet: Barcharts of bovine non-O157:H7 E. coli strain counts (log 10 CFU/mL) in competition assays of high-competitive (HC) and low-competitive (LC) consortia of unique bovine non-O157:H7 strains against E. coli O157:H7 ATCC 700728; Table S1.

    Techniques: Incubation, Competitive Binding Assay, Transformation Assay